Xenbase Image ID: 130566

Figure 1. miR-9 Expression Differs along the AP Axis(A) Whole-mount in situ hybridization for miR-9 expression in X. tropicalis using LNA probe.(B) Expression of miR-9 primary transcripts at stage 30—dorsal view. Dashed line indicates the plane of sectioning in (D). MHB is indicated with an asterisk. Scale bar, 200 μm.(C) Schematic representation of the different regions in the neural tube (red, forebrain; green, midbrain; blue, hindbrain) and transverse sections from the forebrain and hindbrain (red, progenitors; purple, intermediate zone; blue, neurons).(D) In situ hybridization for miR-9 precursors in transverse sections from stage 30 embryos. CNS tissue is outlined with a dashed line. Scale bar, 20 μm.(E) FISH for miR-9a-1 (in red) combined with immunohistochemistry (IHC) for Sox3 (marker for neural progenitors) in stage 30 embryo. CNS tissue is outlined with a dashed line. DNA is stained with DAPI (4,6-diamidino-2-phenylindole). Scale bar, 20 μm. Image published in: Bonev B et al. (2011) © 2011 ELL & Excerpta Medica. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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