Xenbase Image ID: 130567

Figure 2. miR-9 Is Required for Neuronal Differentiation(A) Semiquantitative PCR analysis of mature miR-9 levels in stage 30 WT embryos, injected with miR-9-2 precursor or miR-9 MO at one cell stage. The snRNA U2 is used as a loading control.(B) Experimental outline. miR-9 MO was injected in one cell of the two-cell stage embryo, and the injected side was compared to the control at stage 30.(C) In situ hybridization (whole-mount and transverse sections from the forebrain and hindbrain) with markers for differentiated (N-tubulin) and differentiating neurons (NeuroD1). Note the reduced expression of both markers (arrows) in the miR-9 MO-injected side.(D) Immunohistochemistry on sections for the transcription factor Myt1 indicates impaired neuronal differentiation upon miR-9 knockdown. The FITC tag on miR-9 MO was used to identify the injected side; DAPI was used to stain the DNA.(E) The percentage of Myt1-positive cells in miR-9 MO-injected side relative to the control side in the forebrain (n = 6 embryos, p < 0.001) and hindbrain (n = 9 embryos, p < 0.001). Error bars represent SEM. In all images, scale bars represent 20 μm and CNS tissue is outlined with a dashed line. Image published in: Bonev B et al. (2011) © 2011 ELL & Excerpta Medica. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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