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Figure 4. miR-9 Depletion Negatively Affects the Survival of Forebrain Neural Progenitors(A) TUNEL staining shows increased apoptosis upon miR-9 depletion in the forebrain (arrowheads), but not in the hindbrain.(B) Percentage of the TUNEL+ cells in the injected compared to the control side in the forebrain (n = 6, p < 0.001) and in the hindbrain (n = 6). Error bars represent SEM.(C and D) Sox3-positive domain is expanded in miR-9 MO-injected side when apoptosis is prevented (n = 7, p < 0.001). (E and F) The reduced number of differentiating neurons (Myt1+) upon miR-9 depletion is not rescued by caspase inhibitor block of apoptosis (n = 6, p = 0.003). In all images FITC staining shows the MO-injected side; DNA was counterstained with DAPI. Neural tube is outlined with a dashed line. Scale bars, 20 μm. Error bars represent SEM. Image published in: Bonev B et al. (2011) © 2011 ELL & Excerpta Medica. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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