Xenbase Image ID: 130750

Fig. 4. Overexpression of xPTP-PESTr inhibits gastrulation. Embryos injected in the two blastomeres at two-cell stage were grown to late gastrula (Stage 13) and treated by whole-mount in situ hybridization with the makers Sox2, brachyury (Xbra), and chordin. Embryos expressing the wild-type or the point mutant C231S fail to close their blastopores (white double arrows) and their notochords (n) are shorter than the control (black vertical bar), indicating that gastrulation did not occur properly. In some embryos, two notochord-like structures form around the yolk plug. The neural plate is present (Sox2) but the anterior part is narrower (horizontal black bar) and the posterior portion splits around the yolk plug (y). Image published in: Cousin H and Alfandari D (2004) Copyright © 2004. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue sox2.S anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 13 neural plate pre-chordal neural plate chordal neural plate tbxt.L bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 13 axial mesoderm circumblastoporal collar chrd.L chd, chordin, chrd.1, LOC108716939, X-chordin X. laevis Throughout NF stage 13 notochord axial mesoderm Image source: Published Larger Image Printer Friendly View

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