XB-IMG-130752
Xenbase Image ID: 130752
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Fig. 6. The CCBD is sufficient to mediate xPTP-PESTr-induced cell
spreading. (A) Schematic representation of FN (FN) and the various GSTFN
fusion proteins used in the adhesion assay. The black rectangles
represent the EIIIA and EIIIB domains. The two heparin-binding domains
(HepI and HepII), the V region (V), and the Central Cell-Binding domain
(CCBD) are indicated. The RGD sequence in the 10th type III repeat and
the synergy domain (Syn) in the 9th type III repeats are also marked. The
point mutations in the 9.11a and 9.11e are indicated and the domains
mutated are represented in dark gray. (B) Adhesion assays were performed
as described in Fig. 4 on 10 Ag/ml of fibronectin (FN) or 6 Ag/ml of the
fusion proteins 9.11, 9.11e, and 9.11a. For counting purposes, cells are
considered spread when they display an asymmetrical shape with two or
more lamelliform protrusions. Wt- and C231S drastically increased cell
spreading when compared to GFP, on both fibronectin (FN) and the central
cell binding domain fusion protein (9.11). Cell spreading is never observed
on the mutated fusion protein 9.11a or 9.11e. (C) Histogram representing a
quantitative analysis from four independent experiments for FN and 9.11,
and two experiments for 9.11a and 9.11e. Error bars represent the standard
deviation from the mean. Statistical analysis shows that spreading in
response to wt- and C231S-PTP-PEST is significantly increased compared
to GFP on both FN and 9.11 ( P < 0.01). Image published in: Cousin H and Alfandari D (2004) Copyright © 2004. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |