XB-IMG-130756
Xenbase Image ID: 130756
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Fig. 10. xPTP-PEST perturbation does not affect cell intercalation in vivo. (A) Median optical section of early gastrula stage embryos (Stages 10 to 11)
expressing GFP (top), Wt- (middle), or the C231S-xPTP-PESTr mutant (bottom). The right panels correspond to higher magnification of the blastocoele (b)
roof. The various myc-tagged protein are detected using mAb 9E10 (green) while fibronectin is detected using 32F (red). The yellow color correspond to
regions of overlap. Thinning of the blastocoele roof at the onset of gastrulation down to three cell layers is observed in all experimental cases. (B) Animal cap
expressing either GFP, Wt-, or the C231S-PTP-PEST were cut at Stage 8 induced or not with 5 ng/ml of activin-A and grown until control embryos reached
Stage 15. While all animal cap expressing GFP extend in response to the activin treatment (GFP + activin, black arrowhead), elongation is dramatically reduced
in animal cap expressing either the Wt- or the C231S-PTP-PEST mutant. No extension is observed in the absence of activin ( activin). (C) Optical sections of
tailbud stage embryos (Stage 22) injected in one dorsal vegetal blastomere at the eight-cell stage. Anterior is to the left (Ant) and ventral is down (Vent). The
overexpressed proteins are detected using mAb 9E10 (green), while fibronectin is detected using 32F (red). Cells expressing either the Wt- (left panels) or the
C231S-PTP-PEST (right panels) can participate in dorsal and anterior structures (upper panels), including mesoderm from the head (h), somites (s), and
notochord. The lower panels represent a higher magnification of the notochord (n). The white arrowheads point to the fibronectin staining around the notochord
(left) and the somites (s, right). The staining pattern of green cells alternating with dark cells in the notochord indicates that cells expressing either Wt- (left) or
the C231S-PTP-PEST (right) can intercalate with nonexpressing cells to form the notochord. Image published in: Cousin H and Alfandari D (2004) Copyright © 2004. Image reproduced with permission of the Publisher, Elsevier B. V.
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