Xenbase Image ID: 130818
Fig. 6. BMP-3b and BMP-3 differentially interact with TGF- family members.
Synthetic mRNAs for BMP-2 (A), ADMP (B), Xnr1 (C), derrie`re (D),
and caALK4 receptor (E) were injected into animal caps and their mesoderminducing
activities were challenged by increasing doses of BMP-3b or BMP-3.
Combinations and doses (pg) of each mRNA sample are indicated above lanes.
Animal caps were harvested at stage 12 for scoring by RT-PCR. Brachyury
(XBra) and NCAM served as pan-mesodermal and pan-neural markers, respectively.
A 25-fold excess of BMP-3b (*) interfered with Xbra expression by all
ligands, whereas BMP-3 reduced expression induced by BMP-2 and ADMP.
Both of BMP-3b and BMP-3 alone induced NCAM expression in animal caps
(A), indicating that they are neural inducers.
Image published in: Hino J et al. (2003)
Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V.
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