XB-IMG-130847
Xenbase Image ID: 130847
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Fig. 7. Xpygo-2 and Xpygo-2 antisense morpholinos (MO) cause severe head abnormalities. (A, D, G, J) Head-defective embryos resulting from injection
at the 8-cell stage (DA) with a mixture of 20 ng each of both Xpygo-2 MOs and their corresponding fluorescence views optimized for Lissamine (red
fluorescence, B, E, H, K) to reveal the -2 MO, and fluorescein (yellow-green fluorescence, C, F, I, L) to reveal the -2 MO. Note the association of MO
fluorescence with the head defects seen in the dorsal view (D–F). The coinjection of 1ng each of Xpygo-2 / RNA not targeted by the MOs (J–L) rescued
head structures even in the presence of both morpholinos (K, L). Xpygo-2 MO alone (M, N) caused severe head abnormalities including reduced or absent
eyes and displaced or expanded cement glands (M, arrow). Xpygo-2 MO (40 ng per embryo; O, P) generated only eye deficits that ranged from reduction
and ventral deficits to almost complete loss. Control MO (green fluorescence; 40 ng per embryo; Q, R, lateral view; S, T, dorsal view) did not affect
development. Scale bars: 1 mm. Image published in: Lake BB and Kao KR (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |