XB-IMG-130952
Xenbase Image ID: 130952
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FIG. 6. Loss-of-function analysis; intact embryos. Fertilized eggs
were injected with either (A) 600 pg of ASO281, (B) a mixture of 600
pg ASO281 and 100 pg of XAP-2* RNA, or (C) 600 pg ASO281 with
50 pg each XK81 and XK76, and cultured to stage 20. Control,
uninjected embryos are shown in panel D. ASO281 treatment
resulted in extensive gastrulation failure in 29/29 of the embryos,
with pigmented ectoderm collapsed into a small sac. Co-injection
of XAP-2* RNA rescued essentially normal development in about
12/31 of embryos, partially rescued another 9/31, and had little
effect on the remainder. An example of each is shown in panel B.
Keratin mRNA injection had little if effect; 24/27 were essentially
identical to embryos injected with ASO281 alone, although the
remaining three showed some a more moderate phenotype. One of
the three partially normal embryos is shown in panel C (embryo on
the right). Image published in: Luo T et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |