Xenbase Image ID: 130957

Fig. 5. Verification of the transposon insertion site in founder lines 8F and 9F. a: Genomic polymerase chain reaction(PCR) products for both the 5 (right panel) and 3 (left panel) ends of the integration sites for founder line 8F. Genomic DNA samples were collected from several green fluorescent protein (GFP) -positive F1 8F tadpoles (1, 2, 3, 4) and used in PCR reactions to amplify (3 end 8Fa/8Fb 517-bp fragment; 5 end 8Fc/8Fd 797 bp). b: Agarose gel electrophoresis of genomic PCR products for founder line 9F. Genomic DNA prepared from three GFP-positive F1 9F tadpoles (1, 2 and 3) was used to amplify the expected sized fragments using primer pairs 9Fa/9Fb (493-bp product) and 9Fc/9Fd (264-bp product). No products were formed when genomic DNA harvested from either a GFP-positive 8F F1 tadpole (8F) or a GFP-negative 9F F1 tadpole () was used as the template. L  100-bp ladder. Image published in: Yergeau DA et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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