XB-IMG-131057
Xenbase Image ID: 131057
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Fig. 1. The extracellular Xcadherin-11 domain regulates adhesion of CNC cells in the transplantation assay, independently of β-catenin-binding. (A) Wild-type and Xcad-11 deletion constructs (black, transmembrane segment; dots, β -catenin-binding site). (B) GST-β-catenin pull-down assay. Western blot showing TNT lysates of full-length (X11pcDNA3.1/Myc-His-A) and cytoplasmic-deleted Xcad-11 (δcX11pcDNA3.1/Myc-His-A), all stained with 9E10 Myc antibody (left). Only the full-length Xcad-11 binds the GST-β-catenin fusion protein (right). (C) Transplantation assay. (D) Comparison of cranial neural fold transplants overexpressing different Xcad-11 constructs. Migratory phenotype analysed by GFP fluorescence 18 hours after transplantation. (E) Confocal analysis of transverse transplant sections stained with 9E10 Myc antibody. (F) Transplants expressing the extracellular deletion mutant, δeXcad-11 (δe), started migration earlier than GFP controls. The graph illustrates the comparison of 49 migrating GFP with 46 migrating δeXcad-11 (δe) grafts 0, 7 and 18 hours after transplantation. (G) Lateral views of a transplant expressing δeXcad-11 7 hours after transplantation, showing farther migration compared with the GFP control (left). Dorsal views of the same grafts show no differences after 48 hours (right). Image published in: Borchers A et al. (2001) Copyright © 2001. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |