Xenbase Image ID: 131063

Fig. 7. Homotopic transplants show that non-migrating CNC cells adopt a neural fate. (A) Non-migrating transplant overexpressing δcXcad-11 stained with 2G9 antibody, a neural marker. (B) Migrating GFP-Myc-expressing donor CNC cells (green) are negative for 2G9 (red). (C) Whole-mount in situ hybridisation of a δcXcad-11-expressing graft shows that sox3 is not expressed 1 hour post grafting. (D) GFP-Myc immunostaining of the section shown in C. (E) In situ hybridisation with nrp-1 probe 18 hours after grafting. Donor embryo was injected with δcXcad-11 RNA. (F) GFP-Myc immunostaining of the section shown in E. Upper half of the transplant is nrp-1 positive. (G) In situ hybridisation with twist probe 18 hours after grafting. Donor embryo was co-injected with δcXcad-11 and β-catenin RNA. (H) Section shown in G immunostained with 2G9 (red). Donor embryos were injected either with 1 ng Xcad-11, 2.5 ng δcXcad-11 or 2.5 ng δ cXcad-11 plus 80 pg β-catenin RNA. Asterisks mark the graft centres. Scale bars: 50 μm. Image published in: Borchers A et al. (2001) Copyright © 2001. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page