Xenbase Image ID: 131071

Fig. 5. Construction and effectiveness of a hormoneinducible XTbx5 protein. (A) Schematic diagram of wildtype and dominant negative XTbx5 constructs. The T domain is in red, the engrailed repressor domain is depicted in black and the GR domain is in blue. (B) Wild-type stage 35 embryo with dexamethasone added at the 4 cell stage. (C) Stage 35 embryo injected with 1 ng of XTbx5-EnR mRNA into the dorsal marginal zone at the 4-cell stage. (D) Stage 35 embryo injected with 1 ng of XTbx5-EnR-GR mRNA and no dexamethasone added. (E) Stage 35 embryo injected with 1 ng of XTbx5-EnR-GR mRNA and dexamethasone added immediately after injections. (F) Stage 35 embryo injected with 1 ng of XTbx5-EnR-GR mRNA and dexamethasone added at stage 14/15. In B-F, the inset in the lower left corner shows a single example of a stage 17 embryo. Image published in: Horb ME and Thomsen GH (1999) Copyright © 1999. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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