XB-IMG-131086
Xenbase Image ID: 131086
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Fig. 5. derrière suppresses head formation or
induces a posterior secondary axis in whole
embryos. (A) Experimental scheme. Wild-type or
albino embryos were injected with 50 pg derrière
and 80 pg lacZ RNA in the marginal zone of one
blastomere at 2-cell stage, either dorsally or
ventrally. Albino embryos were harvested at stage
24 (tailbud) for in situ hybridization and wild-type
embryo were harvested at stage 35 (hatching) for
morphological analysis. (B) Dorsal misexpression
of derrière results in microcephaly. In all panels
anterior (A) is to the left and posterior (P) is to the
right. Light blue indicates lineage tracer b-gal.
(a,b) Wild-type embryo injected with derrière
RNA (a) and globin RNA (b). (c-h) Albino
embryos processed for in situ hybridization (see
Results). Purple staining represents probes as
indicated on the left. m-actin is a muscle-specific
marker, otx2 marks the forebrain, XCG marks the
cement gland, en-2 marks the midbrain/hindbrain
junction and Krox20 marks rhombomeres 3 and 5
in the hindbrain. (c-e) White arrow and arrowhead
indicate otx2 forebrain and eye staining,
respectively. Black arrowhead indicates anterior
limit of HoxB9 staining. (f-h) White arrow
indicates en-2 staining, white arrowhead indicates
XCG staining, bracket indicates krox20 staining.
(C) Ventral misexpression of derrière results in a
posterior secondary axis. In all panels, anterior (A)
is to the left and posterior (P) is to the right.
(a,b) Wild-type embryos. White arrow indicates a
secondary axis. (c-j) Albino embryos processed for
in situ hybridization (see Results). Markers as for
B, except for N-CAM, which is a general neural
marker. Arrow indicates secondary axis. (i,j) Arrow
indicates en-2 staining, bracket indicates krox20
hindbrain staining. Image published in: Sun BI et al. (1999) Copyright © 1999. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |