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Fig. 7. Induction of Pitx2 by Xnr1 and activin in animal cap explants. Embryos were injected with goosecoid, activin, Xnr1, eFGF or BMP-4 RNA into animal blastomeres at the 4-8 cell stage, animal caps were cut at stage 8, cultured until gastrula (stage 10.5-11), and assayed for the presence of XPitx2, EF1-a, Xnr1, Xbra and Xvent1 RNA by RT-PCR. Note that Pitx2 mRNA was specifically induced by activin and Xnr1. Co., control uninjected embryos.

Image published in: Campione M et al. (1999)

Copyright © 1999. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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