Xenbase Image ID: 131146

Fig. 6. Vg1 and activin can induce Zic3 in the mesoderm. (A) Zic3 is expressed symmetrically in wild-type embryos. Zic3 expression was enhanced in the mesoderm at stage 10.5 when Vg1 (1000 pg) or activin (5 pg) was injected into the lateral side of one blastomere at the two-cell stage. Embryos were cut along the broken line shown. RT-PCR analysis of animal cap explants from embryos injected with Vg1 (250 pg) (B) or activin (1 pg) (C) at stage 25. Zic3, Xnr1 and Pitx2 are induced by both genes. Vg1 or activin induce the mesodermal marker, m-actin, but not the neural markers, neural celladhesion molecule (N-CAM) and neurogenin. EF1a was used to monitor RNA recovery. Image published in: Kitaguchi T et al. (2000) Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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