XB-IMG-131155
Xenbase Image ID: 131155
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Fig. 2. Expression of Mouse and Xenopus
Hes6 during embryogenesis. (A-F) Hes6 RNA
expression in Mouse embryos. (A) Expression
is first seen at E8.75 in the midbrain
(A, arrowhead) and primordia of sensory
cranial ganglia (arrows). (B) Expression in
DRG is noted at E9.25 (arrow). (C) By E9.5,
Hes6 is expressed in the hindbrain and neural
tube (arrowheads), and expression in DRG is
clearly established (arrow). (D) By E10.5,
extensive expression of Hes6 occurs in the
DRG indicated by arrow and somite by
arrowhead. (E) Expression of Hes6 in the
intermediate zone in the neural tube
(arrowhead; arrow points to DRG) and (F) in
subregions of hindbrain (arrow points to Hes6-
expressing cells and arrowhead indicates
ventricular side) is shown in E10.5 embryo
sectioned following whole-mount in situ
hybridization. (G-J) In situ hybridization of
adjacent sections at the hindlimb level of a
E10.5 mouse embryo. Expression of NeuroD
(H, arrowhead) and Hes6 (I, arrowhead)
overlaps with that of Ngn1 (G) but is highest in
the region adjacent to marginal zone where
terminally differentiated neurons are marked
by SCG10 (J, arrowhead). (K-M) Mouse
embryos at 28-somite stage were probed with
Hes6 (K), NeuroD (L), and Scg10 (M). The
12th DRG for each of the embryo is indicated
by the arrows. Note that expression of Hes6
and NeuroD precedes that of Scg10 by three to
four somites. Note also expression of Hes6 in
the somites at this stage (K; arrowhead).
(N-P) Hes6 RNA expression was localized in
Xenopus embryos by whole-mount in situ
hybridization. (N) Expression of Hes6 in the
nervous system is first detected at open neural plate stages in scattered cells within the three domains where primary neurons form, including a
medial domain (m) corresponding to motoneurons, and a lateral domain (l) corresponding to sensory neurons. (O) By late neurulae stages, higher
levels of Hes6 expression are detected during secondary neurogenesis in the brain (arrow) and eye (arrowhead). Note also the expression of Hes6 in
the tailbud domain (TBD). (P) Tissue section of late neurulae embryo at the level of the hindbrain (otic vesicle: OV) shows extensive Hes6
expression within the intermediate zone of the neural tube along the entire dorsal ventral axis but excluded from the floor plate (fp) and roof plate. Image published in: Koyano-Nakagawa N et al. (2000) Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |