XB-IMG-131223
Xenbase Image ID: 131223
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Fig. 2. Injection of dnSox2 mRNAs suppresses neural
differentiation of animal cap explants caused by
dnBMPR. (A) Expression of N-CAM in normal embryos
at stage 19, when the animal caps below were harvested.
(B-I) Effects of dnSox2 were examined with the N-CAM
marker in the animal cap assay. Injection of dnBMPR (C)
but not of control lacZ (B) mRNA induced N-CAM
expression. The neural differentiation caused by
dnBMPR was inhibited by coinjecting Sox2BD(-)
mRNA (D) or Sox2-EnR mRNA (G). This inhibition was
reversed by Sox2-GR coinjection in the presence of Dex
(E,H) but not in its absence (not shown). The inhibitory
effect of Sox2BD(-) or Sox2-EnR was not counteracted
by Sox9-GR even with Dex treatment (F,I). In each
injection, the total amounts of RNA were made constant
by adding an adequate amount of control mRNA. The
injected amounts of mRNA were 50 pg for dnBMPR,
400 pg for Sox2BD(-), 50 pg for Sox2-EnR, 200 pg for
Sox2-GR, 200 pg for Sox9-GR. Image published in: Kishi M et al. (2000) Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |