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Fig. 4. Injection of Sox2BD(-) inhibits expression of both anterior and posterior neural markers. Whole-mount in situ hybridization analyses were performed with regional neural markers in neurula embryos. (A) SoxDBD(-) mRNA (right) or control lacZ mRNA (left) was injected into each animal blastomere of the 8-cell embryo (400 pg of mRNA/cell). DIG-labeled Otx2 and HoxB9 probes were used simultaneously for double-label in situ hybridization. In the embryo injected with SoxDBD(-), the expression of the forebrain marker Otx2 (arrow) was suppressed whereas that of the spinocaudal marker HoxB9 (arrowhead) was not reduced. (B-F) Embryos were injected with Sox2BD(-) mRNA (right) or control mRNA (left) in a manner comparable to that for SoxDBD(-) mRNA injections. Injection of Sox2BD(-) inhibited expression of Otx2 (B), Xanf1 (C), En2 (D), Krox20 (E), and HoxB9 (F). Note that inhibition of HoxB9 expression was detected only in the posterior neural tube (arrowhead) but not in the posterior mesoderm (arrow). Image published in: Kishi M et al. (2000) Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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