XB-IMG-131226
Xenbase Image ID: 131226
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Fig. 5. Effects of Sox2BD(-) on early neural marker genes.
(A-F) Embryos were injected with Sox2BD(-) mRNA (right) or
control mRNA (left) as described for Fig. 4. Whole-mount in situ
hybridization was performed with Zic2 (A,C,E) and Sox2-5¢UTR
(B,D,F) probes. No significant inhibition of Zic2 or Sox2 expression
was detected at stage 11 (A,B). In embryos at stage 12 and 14,
injection of Sox2BD(-) reduced expression of both early neural
markers (C-F). (G) Accumulation of Sox2BD(-) protein (FLAGtagged)
shown by western blot analysis. Embryos injected with
FLAG-tagged Sox2BD(-) mRNA were harvested at stages indicated
above each lane. Western blotting with anti-FLAG antibody (upper
panel) gave a single band of the expected size (25 kDa) in each lane.
This 25 kDa band was not detected when control lacZ-injected
embryos were used (not shown). CBB staining of SDS-PAGE (lower
panel) is presented as loading controls. Image published in: Kishi M et al. (2000) Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |