XB-IMG-131228
Xenbase Image ID: 131228
Fig. 7. Sox2BD(-) does not promote
epidermogenesis at the cost of neural
tissues. (A) RT-PCR analysis of stage
19 animal caps injected with control
(lane 2), dnBMPR (lane 3), dnBMPR
+ Sox2BD(-) (lane 4) or Sox2BD(-)
(lane 5) mRNA. Primers specific for
N-CAM, epidermal Keratin, XAG-1,
muscle-actin (M-actin) and the
control EF-1a were used.
(B,C) Double-labeled in situ
hybridization with N-CAM (purple)
and Keratin (brown) probes. The
embryos were injected with control
mRNA (B) or Sox2BD(-) mRNA (C)
at one animal blastomere of the 8-cell
embryo. The neural plate region that
failed to express N-CAM (arrow) did not give Keratin signals, either. (D,E) Confocal microscopy images of the neural plate region of neurula
embryos. Green fluorescence shows the progeny of the blastomere that was injected with control lacZ and EGFP mRNA (D) or Sox2BD(-) and
EGFP mRNA (E). The insets are low magnification images showing the nuclear DAPI staining. The brackets indicate the areas shown in high
magnification GFP images. n, notochord; arrows, cuboidal cells in the superficial layer; arrowheads, cells in the sensorial layer. Image published in: Kishi M et al. (2000) Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |