Xenbase Image ID: 131247

Fig. 2. EphB4 signaling is required for directed growth of intersomitic veins. One blastomere of 2-cell stage embryos was injected with RNA encoding tmEphB4 (500 pg; A-D) together with 100 pg RNA of the lineage tracer nuclear b-galactosidase (nucbgal). RNA for tmEphB1 (1000 pg; D) was injected as a control. Embryos were fixed at stage 36, stained for nucbgal and hybridized with Msr. Lateral views are shown with anterior to the left. (A,B) Control (A) and injected side (B) of an embryo. Apart from an aberrant intersomitic vein projecting into an adjacent somite (arrow), the vasculature including the aortic vessels (arrowheads) on the injected side appears overall normal. (C,D) Close-up views of injected sides of embryos. Arrows indicate aberrant projections of intersomitic veins in embryos. A capillary that appears dilated is shown with an arrowhead. Dorsal growth of intersomitic veins is normal in embryos overexpressing tmEphB1. Scale bars: 400 mm (A,B); 200 mm (C,D). Image published in: Helbling PM et al. (2000) Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue aplnr.L agtrl1, angio1, apj, apjr, aplnr-a, aplnr-b, hg11, msr, X-msr, Xangio1, Xmsr X. laevis Sometime during NF stage 35 and 36 to NF stage 40 blood vessel intersomitic vein posterior cardinal vein ophthalmic vein aortic arch duct of Cuvier Image source: Published Larger Image Printer Friendly View

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