Xenbase Image ID: 131249

Fig. 4. Ectopic expression of ephrin-B ligands leads to aberrant intersomitic vein projections. One blastomere of 2- cell stage embryos was injected with either 250 pg of ephrin- B2 (A-D) or with 500 pg of ephrin-B3 RNA (E,F). RNA encoding nucbgal was coinjected as a lineage tracer. Embryos were fixed at stage 36, stained for nucbgal and hybridized with Msr. (A,B) Control (A) and injected side (B) of an embryo expressing ephrin-B2 ectopically. The posterior cardinal vein appears dilated and the intersomitic veins (arrowhead) have failed to grow dorsally. (C,D) Close-up view of control (C) and ephrin-B2 injected (D) trunk regions. Frequently, intersomitic veins are found projecting either abnormally into adjacent somites (arrow) or displaying reduced growth (arrowhead). (E,F) Trunk region of control (E) and injected (F) side of an embryo expressing ephrin-B3 ectopically. Intersomitic veins are disorganized, penetrate aberrantly into the neighboring somites (arrow), and occasionally form loop-like structures (arrowhead). Scale bars: 400 mm (A,B); 200 mm (C-F). Image published in: Helbling PM et al. (2000) Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue aplnr.L agtrl1, angio1, apj, apjr, aplnr-a, aplnr-b, hg11, msr, X-msr, Xangio1, Xmsr X. laevis Sometime during NF stage 35 and 36 to NF stage 40 blood vessel intersomitic vein posterior cardinal vein pronephric sinus duct of Cuvier Image source: Published Larger Image Printer Friendly View

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