XB-IMG-131255
Xenbase Image ID: 131255
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Fig. 4. Confocal imaging of photoactivation marks.
(A) Wide-field digital camera image of a
photoactivation mark in the dorsal region of a stage 31
embryo. (B) Single confocal plane of tailbud region of
stage 31 embryo previously injected with RNAs
encoding membrane-localized and nuclear-localized
GFP proteins (see Materials and Methods). (C) Single
confocal plane of photoactivation mark of embryo in
A. (D) Summed image of confocal z-series of
photoactivation mark in same embryo after horizontal
and vertical edge filters to enhance cell boundaries. 25
images are summed in D. Nine fluorescent cells are
labeled. Note that the single confocal plane in C does
not accurately summarize the entire z series, when
compared to D. Scale bars, 100 mm. Image published in: Davis RL and Kirschner MW (2000) Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |