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Fig. 5. Examples of regional photoactivation. (A,B) Merged fluorescence and DIC images of the same dorsal region photoactivated embryo (TB25-9, Table 1). Anterior is to the left; dorsal is top. Note label in myocytes (Dorsal Myo), notochord (No) and neural tube (NT). (C) Merged fluorescence and DIC image of a dorsal region marked embryo (TB18-1, Table 1) showing label in dorsal fin mesenchymal cells. (D,E) Merged fluorescence and DIC images of the same ventral region photoactivated embryo (TB23-6, Table 2). Anterior is to the right; dorsal is top. Note, E is a higher magnification image of the field of view in D. (F,G) Merged fluorescence and DIC images of the same posterior region photoactivated embryo (TB24-7, Table 3). Anterior is to the left; dorsal is top. Note, G is a higher magnification image of the field of view in F. For ventral and posterior regions, only labeled myocytes (Dorsal Myo and Ventral Myo) are seen (other cells are labeled more posteriorly, but not shown; however, no signal is found in notochord or neural tube in these embryos). Fluorescence is pseudocolored green. Scale bars, 100 mm.

Image published in: Davis RL and Kirschner MW (2000)

Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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