XB-IMG-131289
Xenbase Image ID: 131289
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FIG. 2. Schematic diagram illustrating how the explants were made. (A) Neurula stage 15 embryo. The bold line indicates where the initial
dissection was made to remove the neural plate, cement gland region, and blastopore. This isolated fragment is called whole endoderm plus
mesoderm (WEM). The dashed line indicates where the WEM was bisected to generate the anterior (AEM) and posterior (PEM) explants. (B)
Tail bud stage 25 embryo. As before, the bold line indicates where the head, spinal cord, and tail regions were dissected away. (C) Stage 15
whole endoderm (WE) explant. The WE explants elongate much more than the other explants. Notice, too, that there are no recognizable
organ structures. (D) Stage 15 anterior endoderm (AE) explant. Unlike the WE explants, the AE explants remain as round balls and do not
elongate. (E) Stage 15 posterior endoderm (PE) explant. These explants elongate much more than the AE explants. Image published in: Horb ME and Slack JM (2001) Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |