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XB-IMG-131292

Xenbase Image ID: 131292

FIG. 5. Mesodermal marker expression in neurula stage 15 explants. (A) XNkx-2.5, FoxF1, XTbx5, and xFOG are not detected in the whole endoderm explants. All of these markers are, however, expressed in the explants with mesoderm to various degrees. Notice that XNkx-2.5 is expressed most strongly in the AEM explants. This is in agreement with its endogenous expression pattern in the mesoderm surrounding the duodenum. (B) Neither cardiac actin, a-T3 globin, nor Xtwist is expressed in the whole endoderm explants. Again, expression of each of these markers is seen in the explants with the mesoderm to varying degrees. (C) To confirm that the markers used to detect the presence of mesoderm are indeed expressed in the mesoderm, we examined their expression in explants of isolated anterior or posterior mesoderm from stage-25 embryos.

Image published in: Horb ME and Slack JM (2001)

Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V.

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