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FIG. 7. RT-PCR analysis of vegetal explants. The explants were isolated at late blastula stage 9 and grown in vitro until stage 42. (A) Endodermal marker expression. Xlhbox8 and IFABP, but not Xcad2, are expressed. This suggests that posterior endoderm does not become specified in these vegetal explants. (B) Mesodermal marker expression. All four of the mesodermal markers tested here show expression in the vegetal explants: XNkx-2.5, FoxF1, XTbx5, and xFOG. (C) Expression of previously used mesodermal markers cardiac actin, globin, and Xtwist is absent in the vegetal explants. This confirms that our explants were dissected properly. (D, E) In situ hybridization for FoxF1 (D) and nonexpressed control gene (E) showing scattered positive cells expressing FoxF1 in the vegetal explant. (F) Mesodermal marker expression in mixer-injected animal caps. Mixer-injected animal caps express both XTbx5 and xFOG, but not FoxF1. Uninjected animal caps do not express any of these three markers. Image published in: Horb ME and Slack JM (2001) Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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