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FIG. 8. RT-PCR expression of endodermal markers in mesoderm–endoderm recombinants. (A) Xlhbox8 is only expressed in the PEAM recombinants, and barely in the AEPM recombinants. Conversely, Xcad2 is only expressed in the AEPM recombinants. IFABP is, as expected, expressed in both recombinants. The regional specificity is characteristic of the mesoderm and not the endoderm. (B) To confirm that our mesodermal explants were themselves free from contaminating endoderm, we examined the expression of Edd in anterior endoderm (AE), anterior mesoderm (AM), posterior endoderm (PE), and posterior mesoderm (PM) explants. No expression of Edd is seen in the mesodermal explants, while abundant expression of Edd is seen in the endodermal explants.

Image published in: Horb ME and Slack JM (2001)

Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V.

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