Xenbase Image ID: 131318

Fig. 4. Notch signaling restricts the number of a-tubulin-expressing cells. Albino embryos were injected at 2-cell stage in one blastomere with (A-C) nLacZ RNA alone, (D-F) Notch-ICD and nLacZ RNAs, (G-I) X-Su(H)-DBM and nLacZ RNAs, or (J-L) X-Delta-1Stu and nLacZ RNAs. At stage 17-18 (neurulae), the embryos were fixed, stained in whole mount with X-gal (lightblue reaction product) and then labeled by in situ hybridization for the expression of a-tubulin (dark purple). Left panels show a lateral view of the uninjected side, the middle panels a lateral view of the injected side, and the right panels show a dorsal view. Note that Notch-ICD suppresses a-tubulin expression in 93% of injected embryos (57 embryos in four representative experiments) while the density of a-tubulin-expressing cells increases in embryos injected with X-Su(H)-DBM (55/64 injected embryos) or with XDelta- 1Stu (64/80 injected embryos) in three independent experiments. Image published in: Deblandre GA et al. (1999) Copyright © 1999. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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