Xenbase Image ID: 131319

Fig. 5. Lateral inhibition in the non-neural ectoderm feeds back on X-Delta-1 expression. (A-C) The ventral ectoderm of albinos embryos (stage 11) that were uninjected (A) or injected in one blastomere at the 2-cell stage with RNA encoding Notch-ICD (B) or X-Su(H)-DBM (C) along with nLacZ RNA as a tracer. Embryos were stained with X-Gal (sky blue reaction product) or for the expression of X-Delta-1 using whole-mount in situ hybridization (dark purple). Note that the density of X-Delta-1 expression increases when Notch signaling is blocked (C) and decreases when Notch signaling is increased (B). (D,E) Isolated ectoderm was assayed for the levels of a-tubulin (a-tub) and X-Delta-1 RNAs by RPA. Ectoderm was isolated at stage 9/10 from embryos that were uninjected (uninj.) or injected at 2-cell stage in both blastomeres with RNA encoding Notch-ICD or X-Su(H)-DBM. RNA was extracted at stage 18 or 12 and assayed simultaneously for the levels of a-tubulin and EF1-a RNA, or for X-Delta-1 and EF1-a RNA, respectively. Note that increased Notch signaling (Notch-ICD injection) decreases, while decreased Notch signaling (X-Su(H)-DBM injection) increases the levels of X-Delta-1 and a-tubulin when normalized to recovery of RNA using the levels of EF1-a RNA. RNA from whole uninjected embryos (w. emb.) was used as positive control. Image published in: Deblandre GA et al. (1999) Copyright © 1999. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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