XB-IMG-131321
Xenbase Image ID: 131321
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Fig. 7. XESR-6e restricts the number of
ciliated cell precursors. (A-D) One blastomere
of 2-cell-stage albino embryos was injected
either with 0.5 ng of ESR-6e RNA or 2 ng of
ESR-6eÆb RNA along with nLacZ RNA as a
tracer. At stage 19-20, the injected embryos
were fixed and stained with X-gal (light blue)
and a-tubulin expression by in situ
hybridization (dark blue). Embryos are
oriented with anterior to the left with injected
sides shown in B and D and the uninjected
sides shown in A and C. (A,B) Injection of
ESR-6e RNA suppressed the formation of a-
tubulin-expressing cells in 90% of the embryos
that survived the injection and developed
normally (23 embryos in two independent
experiments) whereas (C,D) injecting RNA
encoding a DNA-binding mutant, ESR-6eÆb,
produced an increase in a-tubulin-expressing
cells in 90% of 48 injected embryos. (E) RPA
on ectoderm isolated from embryos injected
with RNA encoding ESR-6e and ESR-6eÆb
(0.5 and 2 ng respectively) as described in Fig.
5. RNA was extracted from stage 11 animal
caps for assessing X-Delta-1 expression and at stage 16-19 for a-tubulin. Note that ESR-6e decreases the levels of both X-Delta-1 and a-
tubulin RNA while ESR-6eÆb increases their levels. (F) RPA of RNA isolated from ectodermal caps of embryos injected with RNA encoding
wild-type (0.5 ng) and basic domain mutant forms of ESR-6e and ESR-7 (2 ng) as described above. Note that injection of RNA encoding ESR-
6e or ESR-6eÆb leads to a decrease or increase, respectively, in the levels of a-tubulin RNA. Conversely, RNA encoding ESR-7 causes a
decrease in the levels of a-tubulin RNA while RNA encoding ESR-7Æb has no apparent effect. Image published in: Deblandre GA et al. (1999) Copyright © 1999. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |