XB-IMG-131368
Xenbase Image ID: 131368
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Fig. 1. cmXnr2 specifically blocks mesoderm induction by Xnrs. (A) Schematic representation of
putative dominant negative block of Xnr function by cmXnr2 (open box, pro region; shaded box,
mature ligand region). The cleavage site alteration is indicated. TGFb-type molecules form
dimers (disulfide bond indicated). Protease cleavage releases active dimeric ligand; dimerization
of cmXnr2 with Xnrs abrogates this process. (B) cmXnr2 blocks elongation of animal caps
induced by Xnr2 and Xnr4 (stage 25). Parentheses: RNA dose (ng). b-globin was a control RNA.
(C) cmXnr2 blocks mesoderm induction by Xnr1, Xnr2 and Xnr4, but not activin or BVg1 RNA.
RT-PCR analysis of animal cap RNA for the pan-mesodermal marker, Xbra (stage 10.5), and
dorsal mesodermal marker, muscle actin (stage 25). Parentheses: RNA dose (ng). Fibroblast
growth factor receptor (FGFR) RNA was assessed as a loading control. RT(+) and RT(-) indicate
whole embryo RNA transcribed with or without reverse transcriptase (RT). Image published in: Osada SI and Wright CV (1999) Copyright © 1999. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |