Xenbase Image ID: 131374

Fig. 7. cmXnr2 suppresses expression of anterior endodermal markers in vegetal explants. (A) Experimental design. RNAs were injected vegetally into 4-cell embryos, vegetal explants dissected at stage 9 and tested at stage 10.5 or 25 for endodermal marker expression. (B) RT-PCR analysis for early (cerberus, Xhex1, Frzb, Mixer, Xsox17ß and Siamois), and late (endodermin/edd and XlHbox8) endodermal markers. RNA doses injected per blastomere are indicated. cmXnr2-injected VEs were staged by reference to undissected cmXnr2-injected embryos (see text). Suppression of anterior endodermal markers by cmXnr2 was abolished by coinjected Xnr2 RNA. Note that Siamois expression was not affected by cmXnr2. Image published in: Osada SI and Wright CV (1999) Copyright © 1999. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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