XB-IMG-131393
Xenbase Image ID: 131393
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Fig. 1. Effects of Xrx1 overexpression. (A-C) Embryos microinjected
with Xrx1 RNA in the right dorsoanimal blastomere at 8-cell stage.
The arrows point to ectopic pigmented retina located between eye
and diencephalon. (B,C) Transverse sections of an embryo similar to
the one shown in A; (B) a section where the ectopic pigmented retina
is visible (arrow); (C) a more posterior section where the duplication
of the neural tube becomes evident (arrowhead). (D-O) Whole-mount
in situ hybridization analysis of embryos microinjected with Xrx1
RNA at 8-cell stage in one dorsoanimal blastomere. (D-K¢) The
staining pattern of the gene of interest (blue) on the injected side (i)
should be compared with that on the uninjected control side (c). In
the same panels, the distribution of Xrx1-injected RNA is visualized
by cohybridization with Xrx1 antisense RNA revealed by magenta
staining. (M-O) Nuclear b-gal RNA has been used as a tracer and the
b-galactosidase activity is represented by the red staining.
(D-F) Expression of Xpax6 (blue) in Xrx1-injected embryos.
(D) Xpax6 expression at stage 13 is not significantly affected by Xrx1
overexpression; (E,F) Xpax6 expression in the injected and control
side of a stage 23 embryo, respectively. Note that the normal Xpax6
gap of expression in the midbrain present in the control side (area
between lines) has been filled by ectopic Xpax6 expression in the
injected side of the embryo. (G-I) Expression of Xsix3 (blue) in
Xrx1-injected embryos. (G) Expression of Xsix3 at stage 13 is not
affected by Xrx1 RNA injection. (H,I) Xsix3 expression in the
injected and control side of a stage 23 embryo, respectively. The eye
expression of Xsix3 in the injected side appears expanded
dorsoposteriorly when compared to the control side.
(J,K,K¢) Expression of Xotx2 (blue) in Xrx1-injected embryos.
(J) Expression of Xotx2 at stage 13 is repressed by Xrx1
overexpression. (K,K¢) Examples of Xotx2 expression in stage 23
Xrx1-injected embryos where Xotx2 expression is extended laterally
(K) or posteriorly (K¢) in the injected side. The white line marks the
posterior boundary of Xotx2 expression in the control side.
(L) Double whole-mount in situ hybridization performed on a stage
13 normal embryo showing the complementarity of Xrx1 (magenta)
and Xotx2 (blue) expression domains. (M,N) Expression of XAG-1
(blue) in Xrx1-injected embryos. (M) Expression of XAG-1 is
repressed at stage 13 by Xrx1 overexpression. (N) XAG-1 is
ectopically activated in the injected side of a stage 23 embryo.
(O) Expression of XBF-1 (blue) in Xrx1-injected embryos at stage
13. XBF-1 expression is expanded laterally on the injected side. Image published in: Andreazzoli M et al. (1999) Copyright © 1999. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |