XB-IMG-131395
Xenbase Image ID: 131395
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Fig. 3. Effects of Xrx1-EnR RNA microinjection on eye and anterior
head development. (A) Schematic diagrams of Xrx1 constructs used
in microinjection experiments. The homeodomain (Hd), OAR
domain (OAR) and engrailed repressor domain (EnR) are indicated
as well as the amino- and carboxy-termini of the proteins (N and C,
respectively). (B) Stage 41 embryos resulting from microinjection of
400 pg Xrx1-EnR RNA in both dorsoanimal blastomeres at 8-cell
stage. The top embryo is an uninjected control. (C) Xrx1 rescues the
Xrx1-EnR phenotypes. Embryos were coinjected with 400 pg of
Xrx1-EnR RNA and 600 pg of Xrx1 RNA per blastomere in both
dorsoanimal blastomeres at 8-cell stage and analyzed at stage 41.
(D,E) Expression of Xrx1 in presumptive forebrain regions of stage
12.5 normal embryos. (D) Double whole-mount in situ hybridization
with Xrx1 (magenta) and the forebrain-specific marker XBF-1 (blue).
Xrx1 expression partially overlaps with that of XBF-1. (E) Control
embryo hybridized with Xrx1 alone (blue). Image published in: Andreazzoli M et al. (1999) Copyright © 1999. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |