Xenbase Image ID: 131426

FIG. 2. Neural induction is dependent on cortical rotation. (A) RT-PCR analysis of embryos that have been irradiated with UV light (lanes 3–6) and injected radially in the marginal zone at the 4-cell stage with cer-S mRNA (lanes 2 and 4), cer-S and b-catenin (150 pg each) mRNA (lane 5), or cer-S and tBR (1.5 ng) mRNA (lane 6). Note that expression of the neural markers NCAM, Otx-2, Krox-20, and En-2 is inhibited by UV irradiation, but is restored after injection of either b-catenin or a dominant-negative BMP receptor (three embryos per sample; three independent analyses). (B, C) Histological analysis of embryos injected with cer-S mRNA at stage 42. UV treatment results in the loss of neural structures in cer-S-injected embryos (n 5 81). fb, forebrain; hb, hindbrain. Image published in: Wessely O et al. (2001) Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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