Xenbase Image ID: 131429

FIG. 5. The b-Catenin pathway is sufficient to induce preorganizer gene expression program at the blastula stage. (A–D) Embryos were injected into the animal pole at 4-cell stage with synthetic mRNA for b-catenin (150 pg per blastomere), dnGSK-3 (150 pg per blastomere), or were treated with LiCl and analyzed at stage 9 for chordin expression by in situ hybridization. The patch of expression of chordin in (A) marks the position of the preorganizer on the dorsal margin of blastula embryos. All embryos are shown in animal view. (E, F) Ectodermal explants of the embryos treated the same way as above were isolated at stage 8 and the expression levels of chordin, noggin, follistatin, and Xnr3 (E) or cerberus, dkk-1, frzb-1, and gsc (F) were determined by RT-PCR at stage 9. EF1a and ODC serve as control for equal loading. Note that organizer gene markers in (E), but not those in (F), were induced by the b-Catenin pathway in ectodermal explants at blastula. Image published in: Wessely O et al. (2001) Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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