Xenbase Image ID: 131455

FIG. 3. Embryos expressing green fluorescent protein from the Xenopus light chain 2 (XMLC2) and cardiac actin promoters. (A–C) Transgenic embryos expressing GFP from the XMLC2 promoter. (A) Stage 32 embryos stained for GFP RNA expression by whole-mount in situ hybridisation. (B) Section through the heart of the embryo shown in A. (C) Live stage 40 embryo expressing GFP from the XMLC2 promoter. GFP fluorescence in the beating heart is clearly visible at this stage. (D–G) Transgenic embryos expressing GFP from the cardiac actin promoter. (D) Stage 32 embryo stained for GFP RNA expression by whole-mount in situ hybridisation. Note expression in the heart (h) and somites (s). (E) Section through the heart of the embryo shown in D. (F) Live stage 42 embryo expressing GFP from the cardiac actin promoter. GFP fluorescence is clearly visible in the somites (s), heart (h), and muscles of the mouth (m). (G) View of the heart region in a live stage 45 tadpole, expressing GFP from the cardiac actin promoter. At this stage, GFP fluorescence allows the fine structures of the ventricle (v) and outflow tract (oft) to be visible. The gallbladder (gb) exhibits autofluorescence under GFP illumination. Image published in: Breckenridge RA et al. (2001) Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page