XB-IMG-131456
Xenbase Image ID: 131456
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FIG. 4. Expression of BMP4 throughout the heart tube randomises cardiac situs. (A and B) Ventral views of GFP fluorescence in stage 40
transgenic embryos expressing BMP4 from the XMLC2 promoter and GFP from the cardiac actin promoter. (A) Dextrocardia, with a
right-sided ventricle (v), and (B) levocardia, with a left-sided ventricle (v). (C) Transverse section though the middle of an embryo expressing
BMP4 from the XMLC2 promoter, showing an intact notochord (n). (D) RT-PCR analysis of embryos transgenic for BMP4 shows that
transgene expression is seen in embryos exhibiting levocardia (lanes under the red line) and dextrocardia (lanes under the purple line). (1)
Plasmid control, (2) blank control, (g) GFP1 embryo not transgenic for BMP4. (E and F) Right and left views, respectively, of a wild-type
stage 32 embryo stained for Pitx2 expression by whole-mount in situ hybridisation, showing left-sided expression. (G and H) Right- and
left-sided views of a transgenic stage 32 embryo expressing BMP4 from XMLC2 promoter (blue stain), showing unaltered left-sided
expression of Pitx2 (purple stain). (I and J) Right- and left-sided views of a transgenic stage 32 embryo expressing BMP4 from the cardiac
actin promoter (blue stain) in the heart and somites (s). The embryo appears generally abnormal, with Pitx2 (purple stain) expressed
bilaterally, but predominantly on the right, suggesting randomisation of the left–right axis. Image published in: Breckenridge RA et al. (2001) Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |