XB-IMG-131457
Xenbase Image ID: 131457
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FIG. 5. Expression of noggin throughout the heart tube prevents
dextral cardiac looping, but does not affect chamber specification.
(A) Stage 34 embryo expressing noggin from the cardiac actin
promoter and stained for noggin (blue) and cardiac actin (purple) by
double whole-mount in situ hybridisation. Note normal morphology
of embryo. (B) Stage 40 double-transgenic embryo expressing
both GFP and noggin in the heart and somites from the cardiac
actin promoter. Note the developing morphology of chambers in
the unlooped heart. For comparative wild-type stage views see Figs.
3C and 3F. Atrium (a), ventricle (v), outflow tract (oft). (C) Nontransgenic
stage 36 embryo stained for endogenous cardiac actin
RNA by whole-mount in situ hybridisation. Note left-sided ventricle
(v), relative to the position of the outflow tract (oft). (D) Stage
36 transgenic embryo expressing noggin from the cardiac actin
promoter and stained for noggin expression by whole-mount in situ
hybridisation (dark blue stain). Note the persistence of an unlooped
heart with the atrial precursor area (a) caudal to ventricular
precursor (v). Also note the presence of an atrioventricular constriction.
(E) Ventral view of nontransgenic stage 35 whole-mount in
situ hybridisation to XTbx5 antisense RNA probe. XTbx5 is a
transcription factor expressed predominantly in the atrial precursors
and venous pole of the heart. Note sinus venosus (sv) staining.
(F) Stage 35 transgenic embryo expressing noggin from the cardiac
actin promoter and stained for noggin (light blue stain) and Tbx5
(purple stain) by whole-mount double in situ hybridisation. Image published in: Breckenridge RA et al. (2001) Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |