XB-IMG-131464
Xenbase Image ID: 131464
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Fig. 2. Geminin suppresses epidermal and expands neural gene
expression. (A) Synaptobrevin (Knecht et al., 1995; Richter et al.,
1988) expression in the olfactory placode is expanded on the
geminin-injected side of the embryo (15 pg full-length RNA
injected). (B,C) otx 2 expression on geminin-injected (B) or
uninjected (C) sides of the same embryo. In B, otx 2 expression is
expanded posteriorly and the otic vesicle and branchial arches fail to
form normally. (D-F) In situ hybridization for twist (aqua) and
injected geminin (pink). Injection of 250 pg of Ngem RNA
eliminates twist staining (D,E), whereas injection of 25 pg of Ngem
expands twist expression on the injected (right) side relative to the
control half (F). (G-I) Animal hemisphere views of late gastrulae
stained to detect epidermal keratin (aqua) and either 250 pg injected
Ngem (G,H, pink stain) or 250 pg injected GFP (I, pink stain). In G
and H, epidermal keratin staining is lost in injected cells; in I,
overlap of GFP and keratin produces a dark purple stain. Image published in: Kroll KL et al. (1998) Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |