XB-IMG-131470
Xenbase Image ID: 131470
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Fig. 8. A dominant negative geminin induces epidermis and
suppresses neural development in dorsal cells. (A-D) In situ
hybridization to detect epidermal keratin (pink) and injected geminin
(aqua; endogenous not visible); overlap produces a dark blue-purple
stain. Embryos are stage 12. (A,B) Dorsal views of embryos injected
with the geminin C-terminal domain expression plasmid (pCdim).
(C,D) Embryos injected with a plasmid encoding both Cdim and fulllength
geminin (pCdim-gem; 100 pg) and with a plasmid encoding
full-length geminin (pCMVgem; 100 pg). A remaining keratinexpressing
dorsal cell is indicated by an arrowhead. (E,F) In situ
hybridization to detect N-tubulin (dark blue in F) and ectopically
expressed geminin (pink) in transgenic embryos expressing either
pCdim (E) or pCdim-gem (F). Embryos in E, F are stage 23/24. Image published in: Kroll KL et al. (1998) Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |