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Figure 4. The Timing of the Phosphorylation Shifts of Cdc27 and MP44 Is Unaffected by Incubation in Interphase Extracts before Activation by Cyclin B Labeled, in vitro-translated substrates were incubated in interphase extracts for 0 min, 5 min, 10 min, 20 min, or 40 min (numbers above the panel) prior to addition of GST-cyclin B to drive the extracts into mitosis. The reactions were incubated at room temperature and samples were withdrawn for gel analysis at the indicated times (numbers between the panels). Image published in: Georgi AB et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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