Xenbase Image ID: 131505

Figure 5. The Timing of Changes in Nuclear Morphology Is Similar Whether Interphase Nuclei Are Added to Interphase Extracts or to Extracts at Different Times during Mitosis Interphase Xenopus nuclei were added to extracts + rhodamine tubulin at the same time as GST-cyclin B or at 10 or 30 min post cyclin addition. At 4 min intervals, 1 μl of extract plus nuclei was removed, fixed, and stained for microscopic examination. Nuclei at each stage were counted for every time point. (A) Cdc25 phosphorylation shift indicating that the G2/M transition in this experiment occurred 8 min after addition of cyclin B. (B) Graph showing the percent of nuclei at the stage of nuclear envelope breakdown for each time point. The solid vertical line represents the relative time at which the nuclei in each of the experiments were first exposed to a mitotic extract (8 min for the 0 min experiment, and 0 min for the 10 min and 30 min experiments). Open squares, 0 min; closed diamonds, 10 min; closed circles, 30 min; time of addition of nuclei to the extract following cyclin B addition. Image published in: Georgi AB et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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