XB-IMG-131533
Xenbase Image ID: 131533
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Fig. 2. Loss of Xvent gene function induces secondary embryonic
axes. Embryos at the 4- to 8-cell stage were microinjected (A-D)
radially or (E-L) ventrally with the RNAs indicated. The amount of
RNA in ng/blastomere is given in parenthesis. Microcephaly caused
by microinjection of (A) EveXvent-1 (0.1) (47%, n=62) or (B)
EveXvent-2 (0.1) (46%, n=67). Embryos microinjected with control
construct mRNA EveXvent-1(fs) (0.25) were normal (n=44).
Microcephalic phenotypes caused by wild-type mRNA injections of
(C) Xvent-1 (0.15) and (D) Xvent-2 (1) are shown for comparison.
Secondary axis formation resulting from injections of (E) VPXvent-1
(0.1) and (F) VPXvent-2 (0.5). Rescue of secondary axis formation
by mRNA (G) coinjection of VPXvent-1 (0.1) and Xvent-1 (0.15) as
well as (H) coinjection of VPXvent-2 (0.5) and Xvent-2 (1).
(I) Embryos microinjected with Xvent-1P(40) (2.5) mRNA are
normal. (J) Embryo microinjected with Xvent-2P(40) (2.5) showing
secondary embryonic axis. (K) Normal embryo resulting from
mRNA injection of control construct VPXvent-1(fs) (0.5).
(L) Rescue of secondary axis formation by mRNA coinjection of
Xvent-2P(40) (2.5) and Xvent-2 (1). Image published in: Onichtchouk D et al. (1998) Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |