XB-IMG-131534
Xenbase Image ID: 131534
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Fig. 3. VPXvents do not interfere with mesoderm
induction by activin in animal caps. Embryos were
microinjected animally with lacZ (0.5 ng/blastomere),
VPXvent-1(0.1 ng/blastomere) or VPXvent-2 (0.5
ng/blastomere) with or without activin RNA as indicated.
Animal caps were cut at stage 8-9 and cultivated until
sibling embryos reached stage 20. In this experiment,
ventral control injections with both VPXvent-1 and
VPXvent-2 induced more than 50% secondary axes.
(A) Coinjection of VPXvent constructs does not inhibit
elongation of animal caps, induced by activin (0.1
ng/blastomere; top; 100% elongation activin alone (n=17),
100% elongation VPXvent-1 + activin (n=15), 89%
elongation VPXvent-2 + activin (n=18)). VPXvent
constructs alone do not induce elongation of animal caps
(bottom; lacZ, n=13 ; VPXvent-1, n=16; VPXvent-2,
n=16). (B) VPXvent constructs do not inhibit activininduced
mesodermal marker expression in animal caps.
Total RNA was isolated from animal caps at stage 20 and
analysed by RT-PCR for expression of marker genes
indicated. Low and high activin correspond to 0.07 and
0.1 ng/blastomere. Image published in: Onichtchouk D et al. (1998) Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |