Xenbase Image ID: 131535

Fig. 4. Characterisation of secondary embryonic axes. Embryos were microinjected ventrally at the 4- to 8-cell stage with VPXvent-1 (0.1 ng/blastomere), VPXvent-2 (0.5 ng/blastomere) or tBR RNA (0.25 ng/blastomere) as indicated. (A-C) Immune whole-mount staining of embryos with muscle-specific antibody 12-101. Note that all secondary embryonic axes contain muscle. (D-F) Histological analysis of VPXvent-1/2 and tBR-induced secondary embryonic axes. (D) VPXvent-1-induced secondary axes containing notochord. (E) VPXvent-2-induced secondary embryonic axes typically contain no notochord. (F) tBR-induced secondary embryonic axes typically contain no notochord. mu, muscle; no, notochord. Image published in: Onichtchouk D et al. (1998) Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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