XB-IMG-131536
Xenbase Image ID: 131536
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Fig. 5. Loss of Xvent-1/2 function dorsalizes ventral mesoderm. Top,
schematic drawing of VMZ assay. Embryos were either non-injected
(Embryo, DMZ, Co), or injected radially at the 4- to 8-cell stage with
mRNAs encoding VPXvent-1 (0.1 ng/blastomere), VPXvent-2 (0.5
ng/blastomere) and tBR (0.25 ng/blastomere). Dorsal (DMZ) or
ventral (VMZ) marginal zones were explanted at early gastrula stage
and incubated until sibling embryos reached stage 25. Total RNA
was isolated and analysed by RT-PCR assays for expression of
marker genes indicated. Histone H4 was used for normalisation. Image published in: Onichtchouk D et al. (1998) Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |