Xenbase Image ID: 131537

Fig. 6. Analysis of Xvent loss-of-function in animal caps. Top, schematical drawing of animal cap assay. Embryos were either noninjected (Embryo, Co), or injected animally at the 4- to 8-cell stage with the RNAs indicated. Animal caps were explanted at early gastrula stage and incubated until the indicated stage. (A) Loss of Xvent-1/2 function neuralises ectoderm. Doses in ng/blastomere were 0.1 VPXvent-1 RNA, 0.5 VPXvent-2 RNA and 0.25 tBR RNA. Total RNA was isolated at stage 31 and analysed by RT-PCR for expression of marker genes indicated. (B) Induction of organizerspecific genes in animal caps. Doses in ng/blastomere were 0.1 VPXvent-1 RNA and 0.5 VPXvent-2 RNA. Total RNA was isolated at early gastrula stage and analysed by RT-PCR for expression of marker genes indicated. Histone H4 was used for normalisation. Image published in: Onichtchouk D et al. (1998) Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page